Twitter's net loss grew by more than $100 million in the first quarter, though the company's operating earnings and revenue beat Wall Street estimates.
Shares fell 9 percent in after-hours trading.
Monthly active users hit 255 million, up 5.8 percent from the previous quarter but not enough to satisfy investors increasingly concerned about Twitter's struggle to gain a mass following.
The San Francisco-based company also said viewers refreshed their "timelines" - Twitter's equivalent of Web pageviews - 157 billion times in the first quarter, slightly below what some analysts had hoped for.
The company broke even, excluding items, and reported revenue of $250 million.
Analysts had expected the company to report a loss, excluding items, of 3 cents a share on $241 million in revenue, according to a consensus estimate from Thomson Reuters.
The net loss grew to $132.4 million, or 23 cents per share, from $27 million or 21 cents per share a year earlier.
For the current quarter Twitter forecast revenue of $270 million to $280 million and adjusted operating earnings of $25 million to $30 million. Analysts on average expected revenue of $272.9 million and adjusted operating earnings of $28.8 million.
Analysts hold mixed opinions of the stock — 11 of 31 of the investment analysts polled by Thomson Reuters rate it a "sell," outnumbering the seven who deem it a "buy."
The social media tool is popular around the world — some credit it with being vital to protest movements such as Arab Spring — but it has also run into obstacles in international markets. Twitter was briefly banned in Turkey by that country's prime minister, Recep Tayyip Erdogan, who wants the company to open an office there and pay Turkish taxes.
The site is also banned in China, where its Chinese-born competitor, Weibo, thrives in one of the world's largest markets. Weibo recently made an initial public offering on the NASDAQ exchange.
The company did enjoy a recent legal victory at home, though, when a U.S. judge dismissed a case alleging the social media firm committed fraud to fuel interest in its IPO.