Protesters rallied in Savannah, Georgia, where hundreds marched from Johnson Square to Chippewa Square.
"If you're not a woman, you shouldn't be making laws about women's rights," one of them, Daniela Rodriguez, told NBC affiliate WSAV.
Another protester, Nora Cook, said: "I had my first abortion when I was 23. I was an addict at the time, and that abortion gave me the grace of God I needed to get sober, and I'm now about to graduate because of that abortion.
"I can't imagine a world where there are people who would not have that chance," Cook said.
Alabama's abortion ban and some of the other recent restrictions are intended to challenge Roe v. Wade, the landmark 1973 Supreme Court case that legalized abortion nationwide.