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One brother was free on bond and the other remained in jail Wednesday after being charged in connection with the disappearance of Tennessee nursing student Holly Lynn Bobo.

Jeffrey Kurt Pearcy, 42, was released on $25,000 bond after he pleaded not guilty Wednesday to tampering with evidence and accessory after the fact in the presumed death of Bobo, the cousin of country music star Whitney Duncan.

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Pearcy's brother Mark Lynn Pearcy, 38, who is listed in Tennessee criminal records as a registered sex offender, remained in the Carroll County jail on the same charges.

Both brothers were arrested in late May but weren't arraigned until this week, when their possible connection to the high-profile case became public.

Two other men have been charged with murder and kidnapping in the actual disappearance of Bobo, a nursing student at the University of Tennessee-Martin who would now be 23. She was last seen by her brother outside the family's home in Darden in April 2011, and her body has never been found.

Investigators said in an affidavit that Jeffrey Pearcy showed a video to someone "that will prove to be beneficial in the prosecution of a current ongoing investigation being conducted by the Tennessee Bureau of Investigation."

A family photo shows missing Holly Lynn Bobo, who was last seen in April photo

Jeffrey Pearcy's attorney told NBC station WMC of Memphis that Pearcy has never seen a video of Bobo.

The TBI wouldn't reveal what's in the alleged video. In a short statement it emailed to several news organizations, it said, "We won't stop until we find a sense of justice and closure for the family and friends of Holly Bobo."

— M. Alex Johnson