The 90-minute debate quickly descended into chaos after Trump began to interrupt both Biden and the moderator, Fox News' Chris Wallace. The night didn't get much calmer from there.
Read highlights from Tuesday's debate below:
Trump portrays Biden as both too tough and too weak on crime
During an exchange on racism in America, Trump essentially argued that Biden was too tough on crime and is too weak on crime.
First, Trump condemned Biden for authoring the 1994 crime bill while, in almost the same breath, saying Biden wouldn't even say the words "law enforcement."
Biden later said he stands for law and order if it is applied equally regardless of race or creed.
Trump balks at denouncing white supremacy
In another heated exchange, Trump declined to denounce the far right and white supremacists and told the Proud Boys — a violent, far-right group — to "stand back and stand by."
Trump instead tried to pivot to antifa's being a more serious problem. Biden pointed out that the FBI has said the far right is the biggest threat to the U.S. in terms of domestic violence.
His own FBI director said this month that antifa is an ideology, not a group.
Trump and Biden struggle to stay on topic ... with 15 minutes left
We're still updating live here.
Fact-check: Trump says he took advantage of a tax code Biden could have fixed
During an acrimonious exchange, the president defended the low tax bill he is reported to have paid by suggesting that if Biden wanted him to have not taken advantage of the tax code, he should have acted to fix it during his tenure in the Senate.
"The tax code that put him in a position that he pays less tax than a schoolteacher is because of — he says he's smart because he can take advantage of the tax code. And he does take advantage of the tax code," Biden said.
Trump replied: "But why didn't you do it over the last 25 years? Why didn't you do it over the last 25 years?"
In reality, despite being in Senate for 36 years, Biden was never technically in a position to rewrite the federal tax code.
While in the Senate from 1973 to 2009, Biden was chair of the Judiciary and Foreign Relations committees and had no direct hand in writing tax laws. That's the job of the Finance Committee.
Trump, on the other hand, takes advantage of several loopholes to avoid paying taxes, including some for which he personally lobbied.
Among them is a law passed in 1986 to limit investors not actively involved in a business from taking deductions and attributing losses against their incomes. An "at risk" rule was also added to prevent taxpayers from deducting losses greater than their investments. But Congress largely exempted real estate developers, like Trump.
At the same time that his investments in Atlantic City, New Jersey, were suffering, Trump appeared before Congress in 1991 to advocate for "tax shelters" that would "incentivize" "investment in real estate" to help boost the economy during the recession.
Biden calls Trump racist
"He’s the racist," Biden said in a back and forth over Trump’s decision earlier this year to expand a ban on racial sensitivity training to federal contractors.
Trump said he banned the training "because it's racist" and taught anti-American sentiments, making an explicit appeal to the white identity politics that have become a hallmark of his political career.
Fact-check: Trump's attacks on Hunter Biden for foreign business dealings
Trump and his allies have attacked the former vice president's son Hunter Biden for his foreign business dealings.
Trump echoed one of the biggest claims from the recent Senate GOP Homeland Security Committee's "conflicts of interest investigation" into Hunter Biden — Trump claimed on the debate stage that "the mayor of Moscow's wife gave your son $3.5 million. What did he do to deserve it?"
The report, authored by Republican Sens. Ron Johnson of Wisconsin and Chuck Grassley of Iowa, claimed that Elena Baturina, the former wife of the late former mayor of Moscow, wired $3.5 million to a firm associated with Hunter Biden.
Hunter Biden's legal team told NBC News that Biden had "no interest" in the firm that received the money, so "the claim he was paid $3.5 million was false."
And on the debate stage, Joe Biden said the claim had been "totally discredited."
The Senate GOP-led "conflicts of interest" report largely resurfaced outstanding allegations, specifically as to Hunter Biden's role on the board of a Ukrainian energy company, as well as what the committee called "questionable financial transactions between Hunter Biden and his associates and foreign individuals."
Largely focusing on those optics, the report doesn't say Hunter Biden's work changed U.S. policy. Biden campaign spokesman Andrew Bates slammed the report as an "attack founded on a long-disproven hardcore right-wing conspiracy theory" that Johnson "has now explicitly stated he is attempting to exploit to bail out Donald Trump's re-election campaign."
Head hurt? You're not alone.
'Racist' jab from Biden gets no response
Fact-check: Did Trump lower drug prices?
"I'm cutting drug prices. I'm going with favored nations, which no president has the courage to do, because you're going against Big Pharma. Drug prices will be coming down 80 or 90 percent," Trump said.
"He has no plan for health care," Biden argued. "He hasn't lowered drug costs for anybody."
Brand name drug prices are on the rise, too.
What the candidates discussed when it came to race
Follow along live here.
Trump's anti-China rhetoric led to a surge in pandemic racism against Asian Americans
Throughout the debate, Trump boasted of his record on controlling Covid-19 by taking a tough stand on travel from China, repeatedly referring to the coronavirus as the "China plague."
His rhetoric against China and his calling the virus the China virus has led to surges in bias incidents and hate crimes against Asian Americans. Young Asian Americans, in particular, have reported a surge in racist incidents. Asian American business owners have also cited pandemic-related racism as a reason they were forced to close their businesses.